Generate taxonomically representative libraries from the most challenging samples even with sub-nanogram input volumes.
Conventional whole-genome sequencing (WGS) library preparation kits are suboptimal for metagenomic next-generation sequencing (mNGS) workflows involving low-input or host-depleted specimens. These general-purpose kits typically require ≥10 ng of input DNA, lack microbial bias mitigation, and often necessitate the separate procurement of magnetic beads and indexed primers, introducing variability across runs and increasing protocol complexity.
When extracted nucleic acid concentrations fall below the limits of fluorometric quantification (e.g., Qubit), samples frequently fail post-extraction QC. In such cases, library construction cannot proceed, resulting in unusable samples, aborted workflows, and the loss of sequencing opportunity, impacting both operational throughput and data recovery from limited or irreplaceable specimens.
A Purpose-Built mNGS Solution
The Unison Complete Ultralow DNA NGS Library Preparation Kit was developed to address these limitations directly — so you can:
As a standalone library preparation kit
As a core component of the PaRTI-Seq Complete Kit
Devin Microbial DNA Enrichment Kit
Unison Complete Ultralow DNA NGS Library Prep
Free PaRTI-Seq RUO Analysis
Functional at inputs as low as 10 pg
Proprietary Tn5-based tagmentation for uniform and consistent fragment size
Includes magnetic bead purification and dual-indexed primers
Supplied with all critical reagents in a single lot-controlled format
Purpose-built for host-depleted, trace-volume, and complex samples
Consistent percentages of 21 microorganisms in ZymoBIOMICS Microbial Community Standards (Zymo Research) in various NGS libraries prepared by Unison™ Ultralow DNA NGS Library Prep kit with different DNA inputs (10 pg, 1 ng, 50 g) and PCR cycles.
Reduce sample attrition and avoid rework through high-efficiency library prep from inputs that would fail standard QC thresholds
Maintain result integrity across all clinical specimens, improving pathogen detection rates in critical diagnostic investigations
Preserve experimental continuity in rare or limited specimens and enable low-input pilot studies without workflow adaptation
delivers consistent library construction across input ranges that typically lead to failed QC, abandoned runs, and missed opportunities.
Micronbrane Medical technologies enrich our understanding of microorganisms through innovative metagenomic collection devices, novel host depletion, mNGS-grade reagents, advanced metagenomic sequencing assays, automation instrumentation, plus rapid bioinformatic software.
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