General WGS kits skew microbial representation and undermine accurate taxonomic profiling.
Multi-step purification invites contamination and blurs microbial signals, reducing sensitivity.
mNGS samples often fail QC because their DNA levels fall below Qubit detection limits..
Separate beads and indexed primers increase variability and disrupt workflow consistency.
The Unison Ultralow DNA NGS Library Kits overcome the intrinsic limitations of general WGS kits, enabling accurate, high-yield libraries from low-biomass and host-depleted specimens.
Unison is the library-preparation step for host-depleted, low-biomass, and microbiology samples where standard WGS library prep can fail or distort the microbial profile.
Proprietary Tn5-based tagmentation unites fragmentation and adapter attachment in one enzymatic step, minimizing handling, contamination risk, and amplification bias while preserving authentic microbial composition.
Saturation-based normalization maintains controlled insert sizes and even representation across variable input levels—including DNA below Qubit detection thresholds—ensuring reproducible downstream analyses.
Unison generates complete, high-fidelity libraries from sub-nanogram inputs, rescuing samples that would otherwise fail post-purification QC.
Designed for small- to medium-scale studies, the manual kits provide reliable library construction from inputs that fall below conventional detection thresholds.
Consistent insert sizes by two step magnetic beads=based purification for various applications
Performance demonstrated from ≥1 ng down to 10 pg input
One-step tagmentation reduces handling and preserves sample integrity
Normalization ensures similar yields across variable DNA inputs ≥1 ng
The Complete Kit consolidates all reagents including enzymes, magnetic beads, and indexed primers for library construction into a single box, reducing variability and simplifying procurement for demanding projects.
Fully automated workflow reduces contamination and human error
Reproducible performance across large-scale sequencing projects
Maintains low-input capability while increasing throughput
Streamlined processing reduces hands-on time and accelerates turnaround
Built for high-throughput environments, the Automated Kit is optimized for the PrepBot™ instrument to standardize results across large batches with minimal hands-on time.
Integrated reagents reduce sourcing complexity and run-to-run variability
Supports low-input and host-depleted samples, lowering workflow failure rates
Maintains throughput while reducing rework and sample attrition
Enables reproducible results across diverse metagenomic applications
Independent benchmarking compared Unison against Illumina TruSeq DNA Nano, Illumina DNA Prep, and NEBNext Ultra II. The studies show that Unison maintains diversity, reproducibility, and taxonomic fidelity at input levels where conventional kits lose performance.
The Comparative Research Summary: Benchmarking results of Unison versus Illumina and NEB kits at ultralow input levels
Unison maintained stable Shannon and Simpson indices down to 1 ng input, whereas both Illumina DNA Prep and NEBNext Ultra II experienced a pronounced drop in diversity
Figure 1. Alpha diversity (Shannon and Simpson indices) across input levels. Unison maintains microbial diversity at inputs down to 1 ng, while NEB and Illumina show substantial declines below this threshold.
Replicate libraries generated with Unison clustered tightly in PCoA analysis, reflecting minimal amplification bias and strong reproducibility. Illumina and NEBNext libraries spread widely, indicating greater variability and reduced consistency.
Figure 2. Beta diversity PCoA plots of replicate libraries. Unison replicates cluster tightly at ≥1 ng inputs, in contrast to the wider spread observed with Illumina and NEBNext.
At 0.1 ng input, Unison preserved phylum-level community structure without overamplification artifacts. Illumina and NEBNext libraries distorted composition, including enrichment of Actinobacteria – a hallmark of low-input PCR bias.
Figure 3. Phylum-level taxonomic composition at decreasing input levels. Unison maintained balanced representation of major phyla down to 0.1 ng, while Illumina and NEBNext showed distortions and loss of fidelity.
Failed library preparation delays reporting, wastes sequencing runs, and wastes samples. Clinical fluids, swabs, and host-depleted extracts rarely allow recollection, making every input valuable. Benchmarking shows that only Unison sustained replicate consistency and taxonomic fidelity at 1 ng.
Unison kits are engineered for mNGS and specifically for complex and host-depleted samples. Only Unison supports:
Generates libraries from as little as 10 pg of DNA and supports double-stranded, high molecular weight DNA (>1 kb) for normalized Illumina-ready fragments.
A one-step tagmentation method reduces handling and preserves input integrity.
Works with DNA from human, mammalian, and microbial samples across all Illumina sequencing platforms.
Integrates fragmentation, adapter addition, and normalization in a single Tn5-based step.
Delivers consistent insert sizes and reliable library yields across diverse DNA inputs with built-in normalization.
When used together with Devin Microbial DNA Enrichment kit, enables pathogen identification from ~5 million reads at 100 bp read lengths, reducing sequencing cost and time
Micronbrane Medical technologies enrich our understanding of microorganisms through innovative metagenomic collection devices, novel host depletion, mNGS-grade reagents, advanced metagenomic sequencing assays, automation instrumentation, plus rapid bioinformatic software.
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