PaRTI-Seq Analysis: Bioinformatics for Metagenomic Sequencing

PaRTI-Seq Analysis is designed to overcome the computational and interpretive challenges by turning complex datasets into clear, actionable results. Each sequencing run can generate millions of reads, including microbial genomes, host DNA, and environmental noise. Without carefully curated databases and robust pipelines, even experienced labs can encounter false positives, missed detections, and inefficient sequencing runs.

For labs evaluating a sample-to-report mNGS workflow

PaRTI-Seq is the analysis and workflow layer for labs that need more than a sequencing run: host depletion, contamination-controlled extraction, low-input library preparation, and standardized pathogen reporting need to work together.

  • Upstream sample prep: pair analysis with Devin Host Depletion when host DNA is the main barrier.
  • Extraction and contamination control: use Devin Microbial DNA Enrichment where reagent background and microbial gDNA recovery affect interpretation.
  • Library prep: use Unison when host-depleted or low-biomass samples need ultralow-input library construction.
  • Economics: the PaRTI-Seq value model supports a 5M-read workflow versus a 20M-read standard workflow and cites up to 75% cost reduction in the MiniSeq model.
  • Analysis turnaround: use approximately 30 minutes per sample only for Clinical Analysis and PaRTIBot contexts; RUO Analysis turnaround is variable.

Discuss your sample-to-report workflow

In research settings, the challenge often lies in interpreting massive datasets and reconciling inconsistent microbial profiles.

In clinical and commercial settings, precision and reproducibility are critical, as analysis errors can undermine diagnostic confidence, delay reporting, and complicate compliance with regulatory standards.

Mis-annotated or contaminated reference sequences

Incorrect pathogen matches due to alignment errors

Host sequences misclassified as microbial reads

Environmental microbes mistakenly identified as clinically relevant organisms

Reduced sensitivity to low-abundance organisms in host-dense samples

Sources of Error in mNGS Analysis

PaRTI-Seq Analysis directly addresses these issues with deeply curated reference databases, validated algorithms, and optimized workflows for both research and clinical applications.

PaRTI-Seq RUO Analysis enables faster, less expensive, and more accurate research by reducing the need for in-house bioinformatics, removing specialized hardware requirements, and providing curated outputs through a simple web interface. Researchers can upload sequencing data and receive reproducible results from any computer in any location, with outputs delivered in standard formats for downstream analysis.

Features

PaRTI-Seq RUO Analysis

Complimentary access for research use

Curated database of more than 1,500 pathogens

Results available in standard formats (.csv, .xlsx)

Secure AWS-based deployment (Singapore region)

Burrows–Wheeler Aligner (BWA) and minimap2 for accurate read mapping

Preset mapping parameters validated on low-biomass human samples

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Reduces the need for in-house bioinformatics or IT infrastructure

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Ensures consistency across datasets with optimized defaults

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Provides access to a rigorously curated pathogen reference database

Benefits

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Enables reproducible analyses from any location with internet access

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Delivers results in universally compatible formats for downstream analysis

PaRTI-Seq RUO Analysis is included with the following kits:

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PaRTI-Seq Lite Kits

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PaRTI-Seq Complete Kits

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PaRTI-Seq Research Kits

PaRTI-Seq Clinical Analysis

PaRTI-Seq Clinical Analysis is designed to support accurate pathogen detection and streamline data handling under regulatory requirements. It can be deployed in two ways:

Secure AWS cloud platform

ideal for laboratories that want scalable access without the burden of local infrastructure. Data is processed within a protected environment that supports compliance with international data protection standards.

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PaRTIBot (on-premise workstation)

a dedicated local PC that runs PaRTI-Seq Clinical Analysis entirely offline, providing full control over data within the laboratory. This option is suited for institutions with strict privacy requirements or restricted internet connectivity.

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Complimentary access for research use

Complimentary access for research use

Complimentary access for research use

Complimentary access for research use

Complimentary access for research use

Complimentary access for research use

Key Features

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Improves diagnostic confidence with validated reference data and QC metrics

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Reduces reliance on dedicated bioinformatics resources

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Scales to support both routine and high-throughput testing environments

Benefits

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Accelerates reporting through automated, structured outputs

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Eases compliance with data protection and regulatory standards

PaRTI-Seq Clinical Analysis is included with the following kits:

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PaRTI-Seq Plus Kits

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PaRTI-Seq Max Kits

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PaRTI-Seq Clinical Analysis Annual Subscription

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