Application Note – Enhancing mNGS with Small Volume Samples

This study emphasize the successful processing of small volume samples using the PaRTI-Seq™ workflow with a modified protocol. Specifically, the Devin™ filter proved effective in processing biospecimens with volumes as low as 50 μL. Additionally, the DNA extraction and NGS library preparation reagents used in the PaRTI-Seq workflow were found to be compatible with low sample inputs, including vitreous humour samples. This suggests that PaRTI-Seq may also be applicable for other scarce samples such as cerebrospinal fluid (CSF).

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